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spoIVA [2019-10-11 09:16:56]
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spoIVA [2019-10-11 09:16:56]

ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via SpoVM
Locus
BSU_22800
Isoelectric point
4.55
Molecular weight
55.01 kDa
Protein length
492 aa Sequence Blast
Gene length
1479 bp Sequence Blast
Function
spore cortex formation and coat assembly
Product
ATPase, basement layer protein for spore coat assembly
Essential
no
Synonyms
spoVP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class I
  • Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

    • Gene

    Coordinates
    2,386,195 2,387,673

    Phenotypes of a mutant

  • the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • uses ATP hydrolysis to drive self-assembly into static filaments PubMed
  • ATP hydrolysis drives polymerization of a nucleotide-free filament PubMed
  • ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore PubMed
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

    • Domains

  • contains a Walker A ATPase domain

    • Modification

  • phosphorylated on Arg-454 PubMed

    • Localization

  • innermost protein of the spore coat basement PubMed

    • Expression and Regulation

    Operons

    Genes
    spoIVA
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed
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    Biological materials

    Mutant

  • BKE22800 (spoIVA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC, downstream forward: _UP4_TAATACCGGTAGACCTCTTT
  • BKK22800 (spoIVA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC, downstream forward: _UP4_TAATACCGGTAGACCTCTTT

    • References

    Reviews

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    Original publications

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